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BioLegend, Inc.

Company: BioLegend, Inc.
Website: www.biolegend.com
Location: San Diego, California, USA
Employees: 500 full-time employees
Industry:
Life Science/Biotechnology
Founded:  June 2002
Introduction: 
BioLegend develops world-class, cutting-edge antibodies and reagents for biomedical research, manufactured in our state-of-the-art facility in San Diego, CA.  Our mission is to accelerate research and discovery by providing the highest quality products at an outstanding value, along with superior customer service and technical support.

Our product expertise covers a diverse set of research areas including Immunology, Neuroscience, Cancer, Stem cells, and Cell Biology. BioLegend's reagents are supported by superior customer service and a quality management system that is certified for ISO 13485:2003. Our aggressive product development program, through technology licensing, collaborations, and internal hybridoma development, produces strategic reagents for use in a variety of applications including:
Flow cytometry and mass cytometry
ELISA and ELISPOT
Immunoprecipitation and ChIP
Western blotting
Immunofluorescence microscopy
Immunohistochemistry
In vitro or in vivo functional assays.
Quantitative Multiplexing
   
Opening:  2 persons
Field: Biochemistry, Molecular Biology, Cell Biology, Immunology
Description: 
1) The trainee will learn how to clone and amplify both heavy and light chain constructs for each recombinant antibody (recAb), confirm the sequences and make additional DNA as need. The trainee will also have the opportunity to learn how to clone variable region from hybridoma cells and might involve with rabbit recombinant antibody development.

2) Process development for recAb expression – the trainee will acquire knowledge and skills on transient transfection and scale up production. The candidate will also have opportunity to optimize the transfection and culture conditions for mammalian cells to improve the yield and quality of each recAb.

3) Antibody purification and buffer formulation – the trainee will be trained on basic antibody purification and qualification. The candidate might have the opportunity on trouble shooting the formulation and storage conditions for certain troublesome clones.